Carla Dray Marassi; Janete da Silva Gonzaga; Paula Ristow; Rachel Ferreira; Leila Souza Fonseca; Walter Oelemann; Walter Lilenbaum
An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (κ> 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.
Key words: Cattle, Mycobacterium avium subsp. paratuberculosis, serology